I had a similar problem when using the application loader wizard... it just quit mid-process.
Ultimately, I had to just use another computer -- in my case, I was given a new laptop by my company. So I used that... and it worked like a charm. Never disconnected again.
Unfortunately I believe the desktop manager software is very fickle with certain types of computers and their USB connections. I can't explain why, but it appears to be very "hit or miss." Just try a laptop or some other PC.
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