We were running 4.1.5 with 1000 users on a single BES with SQL on the same box. Backup consisted of Symantec Live Recovery (or something like that) taking snapshots to a remote server, but never used it for recovery so cannot comment on it's effectiveness. The only thing we had to do after 500 users was to upgrade the RAM to 4GB, but otherwise all ran fine.
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Better to light a candle than to curse the darkness.
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